Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers

Stepwise strategy for generating osteoblasts from human pluripotent stem cells under fully defined xeno-free conditions with small-molecule inducers

Blog Article

Clinically relevant human induced pluripotent stem cell (hiPSC) derivatives require efficient protocols to differentiate hiPSCs into specific lineages.Here we developed a fully defined xeno-free strategy to direct hiPSCs toward osteoblasts within 21 days.The strategy successfully achieved the osteogenic induction of four independently derived hiPSC lines by a sequential use of combinations of small-molecule inducers.The induction first generated mesodermal cells, which subsequently Curling - Bags recapitulated the developmental expression pattern of major osteoblast genes and proteins.Importantly, Col2.

3-Cherry hiPSCs subjected to this strategy strongly expressed the cherry fluorescence that has been observed in bone-forming osteoblasts in vivo.Moreover, the protocol combined with a three-dimensional Internet Log Book (3D) scaffold was suitable for the generation of a xeno-free 3D osteogenic system.Thus, our strategy offers a platform with significant advantages for bone biology studies and it will also contribute to clinical applications of hiPSCs to skeletal regenerative medicine.